Veröffentlichungen

2018V. Richter, M. Piper, M. Wagner, H. Schneckenburger: “Structured Illumination for Live Cell Microscopy,” Proc. SPIE, Vol. 10686, 2018, 10685-93

An experimental setup for super-resolutionmicroscopy by structured illumination is presented, preliminary experiments of nano-beadsand living cells with a resolution around 100 nm are described, andfurther requirements for live cell microscopy are discussed.

Datei
2018V. Richter, P. Weber, M. Wagner, H. Schneckenburger: "3D visualization of cellular location and cytotoxic reactions of doxorubicin, a chemotherapeutic agent", Medical Research Archives, 2018, in press

Previously we reported on the uptake and interaction of cytotoxic doxorubicin in MCF-7 breast cancer cells grown as standard 2-dimensional cell cultures. Now improved experimental techniques – including axial tomography and Light Sheet Fluorescence Microscopy (LSFM) – permit observation of single cells from any side as well as detection of individual layers in multi-cellular spheroids. Therefore, uptake of doxorubicin in the cell nucleus as well re-localization in the cytoplasm at longer incubation times is well documented. Based on a calcein-AM test we could prove high cytotoxicity in 3D cell cultures at 48-96h after incubation. Simultaneously disintegration of cell spheroids and formation of a degradation product became obvious. Fluorescence lifetime imaging microscopy (FLIM) is presently used to distinguish the fluorescence of doxorubicin and its degradation product, and Structured Illumination Microscopy (SIM) is suggested to improve resolution down to about 100 nm.

Datei
2017H. Schneckenburger, V. Richter, M. Piper, M. Wagner: "Laser Illumination in Live Cell Microscopy: Scattering and Structured Illumination", Journal of Biomedical Photonics & Engineering 3(1) (2017) 010304

Two types of laser illumination in live cell microscopy with a focus on the sample or in the aperture plane of the microscope objective lens are distinguished. For the second case two examples are described, namely light scattering microscopy with angular resolution and Structured Illumination Microscopy (SIM) with two interfering laser beams. Appropriate applications include morphological studies of cells undergoing apoptosis and mitochondrial imaging with increased resolution.

Datei
2017V. Richter, S. Bruns, T. Bruns, P. Weber, M. Wagner, H. Schneckenburger: "Axial tomography in live cell laser microscopy", J. Biomed. Opt. 22(9), 091505 (2017)

Datei
2016T. Bruns, M. Bauer, S. Bruns, H. Meyer, D. Kubin, H. Schneckenburger: "Miniaturized modules for light sheet microscopy with low chromatic aberration", J. Microsc. 264(3) (2016) 261-267

Datei
2015T. Bruns, S. Schickinger, H. Schneckenburger: “Sample holder for axial rotation of specimens in 3D Microscopy”, J. Microsc. 260(1) (2015) 30-36

Datei
2013S. Schickinger, T. Bruns, R. Wittig, P. Weber, M. Wagner, H. Schneckenburger: Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet based fluorescence microscopy, J. Biomed. Opt. 18(12) (2013) 126007.

Datei
2016T. Bruns, S. Bruns, H. Schneckenburger: “Observing the 3rd dimension – A simple way to upgrade common microscopes for sample rotation”, G.I.T. Imaging & Microscopy 2 (2016) 28-30.

Datei
2015P. Weber, S. Schickinger, M. Wagner, B. Angres, T. Bruns, H. Schneckenburger: “Monitoring of apoptosis in 3d cell cultures by FRET and light sheet fluorescence microscopy”, Int. J. Mol. Sci. 16(3) (2015) 5375-5385

Datei
2013H. Schneckenburger: Commentary: Assessing FRET using spectral techniques, Cytometry 83A (2013) 896-897.

Datei
2013P. Weber, M. Wagner, H. Schneckenburger: Cholesterol dependent uptake and interaction of doxorubicin in MCF-7 breast cancer cells, Int. J. Mol. Sci. 14 (2013) 8358-8366.

Datei
2013R. Wittig, V. Richter, S. Wittig-Blaich, P. Weber, W.S.L. Strauss, T. Bruns, T.P. Dick, H. Schneckenburger, Biosensor-expressing spheroid cultures for imaging of drug-induced effects in three dimensions, J. Biomol. Screen. 18 (2013) 736-743.

2012B. von Einem, P. Weber, M. Wagner, M. Malnar, M. Kosicek, S. Hecimovic, C.A.F. von Arnim, H. Schneckenburger: Cholesterol-dependent energy transfer between fluorescent proteins; insights into protein proximity of APP and BACE1 in different membranes in Niemann-Pick Type C disease cells”, Int. J. Mol. Sci. 13 (2012) 15801-15812

Datei
2012T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger: “Preparation strategy and illumination of 3D cell cultures in light sheet based fluorescence microscopy”, J. Biomed. Opt. 17(10) (2012) 101518.

Datei
2012M. Wagner, P. Weber, H. Baumann, and H.Schneckenburger: ”Nanotomography of cell adhesion upon total internal reflection fluorescence microscopy (VA-TIRFM)”, J. Vis. Exp. 68 (2012) e4133.

Datei
2012P. Weber, M. Wagner, P. Kioschis, W. Kessler, and H. Schneckenburger: “Tumor cell differentiation by label-free fluorescence microscopy”, J. Biomed. Opt. 17 (10) (2012) 101508.

Datei
2014T. Bruns, S. Schickinger, H. Schneckenburger: "Mikroskopadapter zur Rotation 3-dimensionaler Proben", BioPhotonik 1 (2014) 40-41

Datei
2012H. Schneckenburger, P. Weber, M. Wagner, S. Schickinger, V. Richter, T. Bruns, W.S.L. Strauss, and R. Wittig: “Light exposure and cell viability in fluorescence microscopy”, J. Microsc. 245 (2012) 311-318.

Datei
2014T. Bruns, S. Schickinger, H. Schneckenburger: "Single plane illumination module and micro-capillary approach for a wide-field microscope", J. Vis. Exp. 15 (90) (2014) e51993

Datei
2012H. Schneckenburger, P. Weber, M. Wagner, T. Bruns, V. Richter, S. Schickinger, and R. Wittig: “Multidimensional fluorescence microscopy in live cell imaging - a mini-review”, Photon. Lasers Med. 1 (2012) 35-40.

Datei
2015V. Richter, F. Voit, A. Kienle, H. Schneckenburger: "Light scattering microscopy with angular resolution and its possible application to apoptosis", J.Microsc. 257 (1) (2015) 1-7

Datei
2011H. Schneckenburger, M. Wagner, P. Weber, T. Bruns, V. Richter, W.S.L. Strauss, and R. Wittig: “Multi-Dimensional Fluorescence Microscopy of Living Cells”, J. Biophotonics, 3 (2011) 143-149.

Datei
2010M. Wagner, P. Weber, T. Bruns, W.S.L. Strauss, R. Wittig, and H. Schneckenburger: “Light dose is a limiting factor to maintain cell viability in fluorescence microscopy and single molecule detection”, Int. J. Mol. Sci. 11 (2010) 956-966.

2010P. Weber, M. Wagner, and H. Schneckenburger: “Fluorescence Imaging of Membrane Dynamics in Living Cells”, J. Biomed. Opt. 15 (2010) 046017.

2010B. von Einem, F. Rehn, D. Schwanzar, P. Weber, M. Wagner, H. Schneckenburger, and C. A.F. von Arnim: “The role of low density receptor-related protein (LRP) as a competitive substrate of APP for BACE1”, Exp. Neurol. 225 (2010) 85-93.

2010H. Schneckenburger, M. Wagner, P. Weber, T. Bruns, and R. Wittig: “Tiefenauflösende Mikroskopie an lebenden Zellen – neue Einblicke mit wenig Licht“, GIT-Bioforum 33/2 (2010) 16-18.

2010T, Bruns und H. Schneckenburger: „Mikrotiterplatte mit Heizeinrichtung“ Patentschrift 10 2009 015 869.3-52, Deutsches Patentamt München (2009); erteilt am 04.10.2010.

2009T. Bruns, B. Angres, H. Steuer, P. Weber, M. Wagner, and H. Schneckenburger: “A FRET-based total internal reflection (TIR) fluorescence reader for apoptosis”, J. Biomed. Opt. 14 (2009) 021003.

2009H. Schneckenburger, R. Börret, C. Braxmaier, R. Kessler, P.Kioschis, D. Kühlke, U. Mescheder, W. Schröder und C. Nachtigall: „Dem Energiestoffwechsel von Tumorzellen und Bioreagenzien auf der Spur“, BioPhotonik 2 (2009) 26-28.

2009B. Angres, H. Steuer, P. Weber, M. Wagner, and H. Schneckenburger: “A membrane-bound FRET-based caspase sensor for detection of apoptosis using fluorescence lifetime and total internal reflection microscopy”, Cytometry 75A (2009) 420-427.

2008M. Wagner, P. Weber, W.S.L. Strauss, H.-P. Lassalle, and H. Schneckenburger: “Nanotomography of cell surfaces with evanescent fields“, Advances in Optical Technologies, Vol. 2008, Article ID 254317 (2008) 7 pages. Doi:10.1155/2008/254317.

2008C.A.F. von Arnim, B. von Einem, P. Weber, M. Wagner, D. Schwanzar, R. Spoelgen, W.S.L. Strauss, and H. Schneckenburger: “Impact of cholesterol level upon APP and BACE proximity and APP cleavage“, Biochem. Biophys. Res. Commun. 370 (2008) 207-212.

2008H.-P- Lassalle, M. Wagner; L. Bezdetnaya; F. Guillemin, and H. Schneckenburger “Fluorescence imaging of Foscan® and Foslip® in the plasma membrane and in whole cells”, J. Photochem. Photobiol. B:Biol. (2008) 92 (2008) 47-53.

2008T. Bruns, W.S.L. Strauss, and H. Schneckenburger: “Total internal reflection fluorescence lifetime and anisotropy screening of cell membrane dynamics”, J. Biomed. Opt. 13 (2008) 041317.

2007H.-P. Lassalle, H. Baumann, W.S.L. Strauss, and H. Schneckenburger: „Cell-substrate topology upon ALA-PDT using variable-angle total internal reflection fluorescence microscopy (VA-TIRFM)”, J. Environ. Pathol. Toxicol Oncol. 26 (2007) 83- 88.

2007R. Sailer, W.S.L. Strauss, M. Wagner, H. Emmert, and H. Schneckenburger: “Relation between intracellular location and photodynamic efficacy of 5-aminolevulinic acid-induced protoporphyrin IX in vitro", Photochem. Photobiol. Sci. 6 (2007) 145 -151

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Prof. Dr. Herbert Schneckenburger

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