H. Schneckenburger: “Laser-assisted optoporation of cells and tissues – a mini-review,” Biomed. Optics Express 10(6) (2019) 2833-2888.
von Herbert, Schneckenburger
Laser microbeam techniques are presented, which permit the introduction of molecules or small particles into living cells. Possible mechanisms - including photochemical, photothermal and opto-mechanical interactions (ablations) - are induced by continuous wave (cw) or pulsed lasers of different wavelength, power and mode of operation. Laser-assisted optoporation permits the uptake of fluorescent dyes as well as DNA plasmids for cell transfection, and, in addition to its broad application to cultivated cells, may have some clinical potential.