V. Richter, P. Lanzerstorfer, J. Weghuber, H. Schneckenburger: "Super-resolution live cell microscopy of membrane-proximal fluorophores”, Int. J. Mol. Sci. 21(19) (2020), 7099, doi:10.3390/ijms21197099
von Herbert, Schneckenburger
Here we present a simple and robust experimental setup for super-resolution live cell microscopy of membrane-proximal fluorophores, which is comparably easy to perform and to implement. The method is based on Structured Illumination Microscopy (SIM) with a switchable spatial light modulator (SLM) and exchangeable objective lenses for epi-illumination and total internal reflection fluorescence (TIRF) microscopy. To show the applicability of this approach, both methods are used to measure translocation of the glucose transporter GLUT4 from intracellular vesicles to the plasma membrane upon stimulation by insulin or insulin-mimetic compounds with a lateral resolution around 100 nm and an axial resolution around 200 nm.