H. Schneckenburger, C. Cremer: “Keepig Cells Alive in Microscopy“. Biophysica 5(1) (2025) 1. https://doi.org/10.3390/biophysica5010001.
by Herbert, Schneckenburger
Light microscopy has emerged as one of the fundamental methods to analyze
biological systems; novel techniques of 3D microscopy and super-resolution microscopy
(SRM) with an optical resolution down to the sub-nanometer range have recently been
realized. However, most of these achievements have been made with fixed specimens, i.e.,
direct information about the dynamics of the biosystem studied was not possible. This
stimulated the development of live cell microscopy imaging approaches, including Low
Illumination Fluorescence Microscopy, Light Sheet (Fluorescence) Microscopy (LSFM), or
Structured Illumination Microscopy (SIM). Here, we discuss perspectives, methods, and
relevant light doses of advanced fluorescence microscopy imaging to keep the cells alive at
low levels of phototoxicity.