Prof. Dr. Christian Neusüß

Room

116

Telephone

+49 7361 576-2399

Email Address

Research
Resumé
Publications

The academic study and workplace training; academic knowledge and practical experience; theory and practice of instrumental analytical chemistry and bioanalytics are the main contents of my teaching. These contents are special fields of the masters/master's programme "Analytical and Bioanalytical Chemistry". In addition to advanced courses / specialties in the fundamentals of inorganic, council and physical chemistry, the course focuses on topics in analytics, spectroscopy and biochemistry. As Dean of Studies, Studies Dean of this exciting course, I welcome your interest and will be happy to answer any questions you may have.

In the field (of) research, I am mainly concerned with the coupling of modern separation techniques (CE, LC) with mass spectrometry. As a comparatively rarely practiced School of Engineering, we have built up a great deal of expertise in CE/MS. In addition to fundamental work, this involves a wide range of applications in pharmaceutical analysis, environmental analysis and structure determination. Together with my doctoral candidates, doctoral students and students writing their Masters (UK)/master's (US) thesis or project, we have a lot of fun working in a state-of-the-art laboratory / lab.

Research Projects

Characterization of intact glycoproteins (e.g. EPO) using CE-MS
Method development for the characterization of glycans, based on CE-LIF, CE-MS and LC-MS

Collaboration, cooperation with several (bio)pharmaceutical companies.

Project GEMSE 01.06.2010 - 31.05.201375 KBPDF

The project with the title "Capillary electrophoresis/mass spectrometry - new approach for coupling two important techniques for protein characterization" is funded and supported by the BMBF through the 2010 round of funding for the qualification of young engineers.

Sub-project of MIME 01.11.2011 - 31.10.201469 KBPDF

The sub-project runs under the joint project Multimodal Pattern Recognition System for the Protection of the Population against Organized Drug Crime and for Combating International Drug Trafficking as part of the BMBF's "Securing Commodity Chains" announcement under the Federal Government's "Research for Civil Security" program.

Equipment

Various LC, CE and GC instruments can be coupled with MS, UV, fluorescence, LIF, conductivity detection

Equipment	manufacturer	Variant/Detection
Liquid chromatography*
LC Ultimate 3000	Dionex	LC or capLC
HPLC 1050	Hewlett Packard / Agilen	UV, fluorescence, conductivity detection
HPLC 1100	Agilent	UV, DAD, conductivity detection
HPLC LC-10A VP	Shimadzu	DAD
LC	LDC	Fluorescence (Shimadzu RF-551)

Gas chromatography
GC 5890 Series II Plus	Hewlett Packard / Agilent
GC 5710A	Hewlett Packard / Agilent
GC MCS 5890 Series II	Gerstel
GC-MSD 5890 Series II	Hewlett Packard / Agilent	Quadrupole MS, optional with headspace

Capillary electrophoresis*
CE 7100	Agilent	Optional flexible UV/VIS absorption or LIF at 488nm or 266nm (J+M Analytics)
P/ACE MDQ CE	Beckman Coulter	Optional flexible UV/VIS absorption or LIF at 488nm or 266nm (J+M analytics)
P/ACE System 5500	Beckman Coulter	Optional flexible UV/VIS absorption or LIF at 488nm or 266nm (J+M Analytics)
P/ACE System 5500	Beckman Coulter	Optional flexible UV/VIS absorption or LIF at 488nm or 266nm (J+M Analytics)

Mass spectrometer
Ion trap Esquire 6000	Bruker Daltonics
MicroTOFq-MS	Bruker Daltonics

UV/VIS spectroscopy
UV/VIS spectrometer LKB Ultrospec Plus	Pharmacia

Raman spectroscopy
Raman spectrometer (785nm)	Polytec / J+M Analytics

1989 - today Appointment funding for professors; appointment funds

Henry Frick, Laura Dhellemmes, Alisa Höchsmann, Laurent Leclercq, Christian Neusüβ, Hervé Cottet, Norbert Schaschke ISSN: 1759-9962,

Further information

Jasmin Schairer, Jennifer Römer, Christian Neusüß ISSN: 0731-7085,

Further information

Alisa Höchsmann, Henry Frick, Laura Dhellemmes, Laurent Leclercq, Philipp T. Kaulich, Andreas Tholey, Hervé Cottet, Norbert Schaschke, Christian Neusüß ISSN: 1615-9853,

Further information

Ann-Katrin Schwenzer, Christian Neusüß ISSN: 0003-2700,

Further information

Noah Gould, Qianjie Wang, Jeffrey N. Agar, Jennifer S. Brodbelt, Daoyang Chen, Kellye A. Cupp‐Sutton, Elena Domínguez‐Vega, Fei Fang, Marianne Fillet, Matthew S. Fischer, Attila Gaspar, Ying Ge, Marie‐Jia Gou, Michal Greguš, Christoph Gstöttner, Narmin Hamidli, Amanda Helms, Md Amin Hossain, Kyle J. Juetten, Neil L. Kelleher, Tobias Kraus, Eli J. Larson, J. Scott Mellors, Cynthia Nagy, Christian Neusüß, Erin A. Redman, Jasmin Schairer, Si Wu, Tian Xu, Zhitao Zhao, Guijie Zhu, Alexander R. Ivanov, Kevin Jooß, Liangliang Sun ISSN: 1433-7851,

Further information

Jasmin Schairer, Alisa Höchsmann, Benedikt Bauer, Volker Höfer, Jennifer Römer, Christian Neusüß ISSN: 1942-0862,

Further information

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Prof. Dr. Christian Neusüß

Research

Research Projects

Equipment

Resumé

Dean of Studies, Studies Dean "Analytical and Bioanalytical Chemistry"

Professors / Professor of the academic staff for General and Analytical Chemistry

Research assistant/associate/fellow / application chemist at Bruker Daltonik GmbH

Research assistant/associate/fellow at the Department, Institute for Tropospheric Research

Studied chemistry in Heidelberg (German university degree 1996) and Bergen (Norway)

Publications

Adjusting the electroosmotic flow for CE separation of proteins by using poly(α-L-lysine)-based mixed polycationic/polyzwitterionic multilayer coatings

CE-MS and CE-MS/MS for the multiattribute analysis of monoclonal antibody variants at the subunit level

Enhanced Separation of Intact Proteins and Proteoforms by CZE‐MS Using Sulfobetaine‐Modified Poly(α‐L‐lysine)‐Based Multilayer Coatings for EOF Adjustment

High-Performance Native Separation of mAb Proteoforms by CZE-MS under Native and Denaturing nanoESI Conditions for Ultrasensitive Charge Variant Characterization

Intact Proteoform Analysis by Capillary Electrophoresis–Mass Spectrometry. Are We There Yet?

Ion-exchange chromatography, capillary isoelectric focusing, and capillary zone electrophoresis coupled to mass spectrometry for charge variant analysis of monoclonal antibodies